Anti-Antibody Activity of a Monoclonal Macroglobulin

Anti-antibody activity of a monoclonal macroglobulin.

A monoclonal macroglobulin from a patient with Waldenström macroglobulinemia has been shown to have antiglobulin activity of a new type, namely, that of a true anti-antibody. The protein reacts with human, primate, and rabbit IgM and IgG, only when the target immunoglobulin is complexed to an antigen. The implications of the general findings of antiglobulin activity of monoclonal proteins is di...

Development of a Novel Inhibitory Chimeric Anti-HER2 Monoclonal Antibody

A monoclonal macroglobulin with antinuclear activity.

Serum containing a monoclonal IgM protein from a patient with Waldenstroms' macroglobulinaemia gave intense immunofluorescent staining of kidney nuclei. The Fab mu fragments of this immunoglobulin were obtained. The IgM and Fab fragments reacted in vitro with kidney nuclei using unfixed cryostat sections of rat or mouse kidney. After treatment of the patient with chemotherapy, the monoclonal Ig...

Binding activity of a murine anti-lipid A monoclonal antibody.

In this report we briefly describe an immunoglobulin G3 monoclonal antibody, 2G6/1H11, which binds purified lipid A from Salmonella minnesota and a lipid A precursor molecule derived from Salmonella typhimurium. 2G6/1H11 does not bind well to purified whole S. typhimurium lipopolysaccharide (LPS), S. minnesota LPS, LPS preparations from a series of S. minnesota rough mutants, or intact S. typhi...

99mTc Direct radiolabeling of PR81, a new anti-MUC1 monoclonal antibody for radioimmunoscintigraphy

  Introduction: Monoclonal antibodies labeled mainly with 99mTc are being widely used as imaging agents in nuclear medicine. Recently PR81 was introduced as a new murine anti-MUC1 MAb against human breast carcinoma. This antibody reacts with the tandem repeat of 20-mer peptide of protein backbone, and is of IgG1 class, with an affinity of 2.19×10-8 M-1. Due to high sp...